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CMOS electrochemical pH localizer-imager
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DOI：10.1126/sciadv.abm6815.png)
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Abstract
En 中文
pH controls a large repertoire of chemical and biochemical processes in water. Densely arrayed pH microenvironments would parallelize these processes, enabling their high-throughput studies and applications. However, pH localization, let alone its arrayed realization, remains challenging because of fast diffusion of protons in water. Here, we demonstrate arrayed localizations of picoliter-scale aqueous acids, using a 256-electrochemical cell array defined on and operated by a complementary metal oxide semiconductor (CMOS)-integrated circuit. Each cell, comprising a concentric pair of cathode and anode with their current injections controlled with a sub-nanoampere resolution by the CMOS electronics, creates a local pH environment, or a pH voxel, via confined electrochemistry. The system also monitors the spatiotemporal pH profile across the array in real time for precision pH control. We highlight the utility of this CMOS pH localizer-imager for high-throughput tasks by parallelizing pH-gated molecular state encoding and pH-regulated enzymatic DNA elongation at any selected set of cells.
Keywords:
MICROELECTRODE ARRAY
DEOXYNUCLEOSIDE PHOSPHORAMIDITES
NUCLEOTIDE CHEMISTRY
PEPTIDE-SYNTHESIS
SENSOR ARRAY
ELECTRODES
DESIGN
CHIP
DEPENDENCE
HYDROGEN
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