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Colored point spread function engineering for parallel confocal microscopy
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DOI：10.1364/OE.24.027395.png)
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Abstract
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Using the color selectivity of a spatial light modulator (SLM) for both, tailoring the excitation beam at one wavelength, and multiplexing the image at the red-shifted fluorescence wavelength, it is possible to parallelize confocal microscopy, i.e. to simultaneously detect an axial stack (z-stack) of a sample. For this purpose, two diffractive patterns, one steering the excitation light, and the other manipulating the emission light, are combined within the same area of the SLM, which acts as a pure phase modulator. A recently demonstrated technique allows one to combine the patterns with high diffraction efficiency and low crosstalk, using the extended phase shifting capability of the SLM, which covers multiples of 2 pi at the respective wavelengths. For a first demonstration we compare standard confocal imaging with simultaneous image acquisition in two separate sample planes, which shows comparable results. (C) 2016 Optical Society of America
Keywords:
DIFFRACTIVE PHASE ELEMENTS
SPATIAL LIGHT-MODULATOR
OPERATION
LENSES
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