.png){kind=icon}
.png){kind=icon}
Return
Comparative analysis of ducks liver gene expressions infected with virulent or attenuated DHAV-3 reveals divergent host responses to viruses of different virulence
.png){kind=icon}
OA
AI
DOI：10.3389/fmicb.2026.1740464.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
IntroductionDuck hepatitis A virus type 3 (DHAV-3) causes acute fatal hepatitis in ducklings.MethodsThis study compared host transcriptomic responses in duck livers following infection with virulent (HB) or attenuated (HB80) strains.ResultsRNA sequencing (RNA-Seq) revealed that the virulent HB strain induced 2; 355 differentially expressed genes (DEGs) at 2 days post-infection (dpi); compared to only 322 DEGs triggered by the attenuated HB80 strain. Functional analysis showed that the HB strain robustly activated immune pathways; particularly Toll-like receptor (TLR) and RIG-I-like receptor (RLR) signaling; leading to a potent type I interferon (IFN-I) response and marked chemokine upregulation. In contrast; the HB80 strain elicited a markedly milder immune reaction. Among the DEGs; 77 immune-related genes were identified; with significant enrichment in the IFN-I signaling pathway; suggesting their critical role in initiating an interferon storm and subsequent chemokine upregulation. Selected key genes (IFN-α2; RSAD2; RIG-I; MDA5; TBK1; TLR7) were validated by RT-qPCR and ELISA for targeted protein confirmation.DiscussionOur study findings suggest that paternal care is associated with medical or co-medical professional choices. Further prospective studies are required to determine causal relationships and investigate other factors related to such choices; given the non-association of all other variables in the study.
Keywords:
virulence
immunity
transcriptome
IFN-I
duck hepatitis A virus type 3
Journal
IF:
4.5
Papers:
4.0W
Citations:
16.6W
Organization
View more organizations