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Comparison of antibody functionality using different immobilization methods

delete2003-08-29
delete129
PRE
AI
R
R. Danczyk
B
B. Krieder
A
Adam North
T
Thomas J. Webster
H
Harm HogenEsch
A
Ann E. Rundell *
DOI：10.1002/bit.10760delete
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Abstract

Abstract

En 中文
This study investigates the influence of antibody immobilization methods on antigen capture. Adsorption and two surface chemistries, an aminosilane chemistry and a common heterobifunctional crosslinker (N-gamma-maleimidobutyryloxy-succinimide ester, GMBS), were compared and evaluated for their ability to immobilize antibodies and capture antigen. The role of protein A as an orienting protein scaffold component in each of these techniques was also evaluated. Through experimentation it was determined that the GMBS technique immobilized the highest amount of antibody and minimized nonspecific binding. For all techniques, the most functional antibodies were found to be those immobilized with protein A. Interestingly, the aminosilane technique demonstrated the highest antigen capture with antibody alone but also exhibited the highest level of nonspecific binding. (C) 2003 Wiley Periodicals.
Keywords:
antibody
antigen capture
protein immobilization
protein A
biosensor
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Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Biotechnology and Bioengineering cover
Biotechnology and Bioengineering
IF:
3.6
Papers:
9.8K
Citations:
2.2W

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