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Complement recruitment using bispecific diabodies
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DOI：10.1038/nbt0797-629.png)
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Abstract
En 中文
We describe the engineering of antibody fragments produced in bacteria for recruitment of complement effector functions. From a phage display repertoire we isolated human antibody fragments directed against complement Clq, and linked these to lysozyme-specific antibody fragments, creating bispecific antibodies (diabodies). One diabody was able to recruit Clq, resulting in efficient lysis of lysozyme-coated sheep erythrocytes, and also induced rosette-formation of erythrocytes with human monocytes and phagocytosis after phorbol ester stimulation. These diabodies may have therapeutic applications requiring the activation of complement.
Keywords:
antibody engineering
diabody
complement C1q
phage display
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41.7
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