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Computed Origami Tomography
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DOI：10.1137/20M1378247.png)
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Abstract
En 中文
In this paper, we provide assembly instructions for an easy-to-build experimental setup in order to gain practical experience with tomography. As such, this paper can be seen as a complementary work to excellent undergraduate-level mathematical textbooks concerned with the basic mathematical principles of tomography. Since the setup uses light for tomographic imaging, the objects investigated need to be light transparent, such as origami figures. Should the reader want to experiment with computational tomographic reconstructions without assembling the device, we provide a database of several objects together with their tomographic measurements and publicly available software. Moreover, recent advances in cryo-imaging have enabled three-dimensional high-resolution visualization of single particles such as, for instance, viruses. To exemplify, and demonstrate, single particle cryo-electron microscopy, we provide an advanced assembly that we use to generate data simulating a cryo-recording. We also discuss some of the major practical difficulties in reconstructing particles from cryo-microscopic data.
Keywords:
open source software
educational report
Radon transform
X-ray tomography
cryo-microscopy
data simulation
reconstruction