Construction of a susceptible cell model for decapod iridescent virus 1 (DIV1) infection

Jing Chen

doi:10.1016/j.jip.2026.108649

Construction of a susceptible cell model for decapod iridescent virus 1 (DIV1) infection

2026-05-09

0

PRE

AI

J

Jing Chen

X

Xue-Mei Yuan

L

Lei Huang

X

Xian-Qi Peng

A

A-Qin Zheng

J

Jin-Biao Jiao

J

Jia-Yun Yao *

DOI：10.1016/j.jip.2026.108649

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Abstract

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• We established an in vitro infection model for DIV1 using mosquito cells, systematically determining the optimal virus-cell incubation time to be 6 h post-infection. • DIV1 propagated in the established cell model reached a titer of 10−2.67 TCID50 per 0.1 mL and induced characteristic cytopathic effects, including cell rounding, granulation, and detachment. • Cell-cultured DIV1 retained high pathogenicity in Macrobrachium rosenbergii, causing dose-dependent mortality and severe histopathological damage in the hepatopancreas, with visible viral particles detected in affected tissues. • Complete genome of a DIV1 strain was sequenced (165,567 bp, 34.58% GC, 176 ORFs), confirming its classification within the genus Decapodiridovirus.

Keywords:

DIV1

mosquito cells

cell model

Decapodiridovirus

pathogenicity

Construction of a susceptible cell model for decapod iridescent virus 1 (DIV1) infection

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