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CRISPR-based non-nucleic acid detection
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DOI：10.1016/j.tibtech.2025.04.012.png)
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Abstract
En 中文
Clustered regularly interspaced short palindromic repeats (CRISPR) technology can detect both nucleic acid and non-nucleic acid (NNA) targets. CRISPR-based NNA detection systems use CRISPR-associated (Cas) trans-cleavage as a signal amplifier. The specificity of CRISPR-based NNA detection systems is determined by NNA-targeting strategies, including aptamers, DNAzymes, allosteric transcription factors (aTFs), antibodies, and cofactor-based biochemical reactions. The underlying mechanism of CRISPR-based NNA detection is to link the presence of a target NNA to the accessibility of three essential CRISPR components: the activator, CRISPR RNA (crRNA), and Cas effector. CRISPR-based NNA detection is sensitive, rapid, and free of aerosol contamination risk, and can be used in both clinical and nonclinical scenarios.
Keywords:
CRISPR diagnostics
Cas12
Cas13
trans-cleavage
non-nucleic acids
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