CST-Based Molecular Profiling of Vaginal Microbiota Validated by qRT-PCR and 16S Amplicon Sequencing

Eunhye Lee

doi:10.1016/j.ymeth.2026.03.015

CST-Based Molecular Profiling of Vaginal Microbiota Validated by qRT-PCR and 16S Amplicon Sequencing

2026-04-14

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DOI：10.1016/j.ymeth.2026.03.015

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Abstract

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• Species-specific qRT-PCR primers were developed for CST-associated vaginal bacteria. • A targeted qRT-PCR assay enables rapid and cost-effective vaginal microbiome profiling. • qRT-PCR quantification showed strong concordance with 16S rRNA sequencing data. • The method reliably distinguishes Lactobacillus-dominant and BV-associated communities. • This approach supports practical CST-based analysis without full metagenomic sequencing.

Keywords:

vaginal microbiota

qRT-PCR

CST

16S rRNA sequencing

molecular profiling

CST-Based Molecular Profiling of Vaginal Microbiota Validated by qRT-PCR and 16S Amplicon Sequencing

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