De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly

doi:10.7554/eLife.10586

De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly

2015-11-26

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DOI：10.7554/eLife.10586

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Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur. Consistently, centriole formation is thought to strictly rely on self-assembly, involving self-oligomerization of the centriolar protein SAS-6. Here, through reconstitution of de novo synthesis in human cells, we surprisingly found that normal looking centrioles capable of duplication and ciliation can arise in the absence of SAS-6 self-oligomerization. Moreover, whereas canonically duplicated centrioles always form correctly, de novo centrioles are prone to structural errors, even in the presence of SAS-6 self-oligomerization. These results indicate that centriole biogenesis does not strictly depend on SAS-6 self-assembly, and may require preexisting centrioles to ensure structural accuracy, fundamentally deviating from the current paradigm.

Keywords:

CARTWHEEL-LESS CENTRIOLES

GAMMA-TUBULIN

DUPLICATION

MOUSE

ORGANIZATION

CENTROSOMES

CONVERSION

PATHWAY

CYCLE

De novo centriole formation in human cells is error-prone and does not require SAS-6 self-assembly

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