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Decoding glomalin: Mycorrhizal hyphae-secreted glomalin-related soil protein in a zero-carbon background unveils Hsp60 homology and functional molecular architecture
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DOI：10.1016/j.soilbio.2026.110233.png)
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Abstract
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• The zero-carbon background enabled isolation and collection of hyphae-secreted GRSP. • Purified GRSP contained peptides homologous to Hsp60 and exhibits a network-like microstructure. • DE-GRSP showed higher hydrophobicity and Fe2+ valence than EE-GRSP; both have humic-like spectra. • Fungal Hsp60s were differentially upregulated in symbiosis, and FmHsp60 was induced more strongly. • GRSP was a functional molecule with defined structure, not an inert microbial residue.
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