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Design of experiments-driven optimization of dual ultra-high-performance liquid chromatography high resolution mass spectrometry for integrated fecal metabolomics and lipidomics
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DOI：10.1016/j.aca.2026.346087.png)
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Abstract
En 中文
• Dual column UHPLC-HRMS platform integrates fecal metabolomics and lipidomics in a single workflow. • Multivariate DoE optimization of 13 key LC-MS parameters under dual-operation constraints. • Systematic evaluation of interaction effects beyond one-factor-at-a-time approaches. • 21% shorter analysis time while maintaining or slightly improving targeted compound and untargeted feature detection. • Statistically guided framework for harmonized dual-column LC-MS method development.
Keywords:
Analytical method development
Integrated multi-omics
Stool
Gut phenotyping
Statistical design
Small molecules
Journal
IF:
6
Papers:
3.3W
Citations:
6.1W
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Cited Papers