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Detection of H5 Subtype Avian Influenza Virus in Avian Oropharyngeal Swab Samples Using a Microfluidic Non-Competitive Fluorescence Polarization Immunoassay
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DOI：10.1039/D6AN00505E.png)
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Abstract
En 中文
Rapid and simple methods for on-site detection of avian influenza virus (AIV) are essential for effective field surveillance. In this study; a microfluidic device-based non-competitive fluorescence polarization immunoassay (NC-FPIA) using an ATTO 647N-labeled hemagglutinin fragment of H5-AIV as a tracer was developed for the quantification of H5 subtype AIV (H5-AIV) in avian oropharyngeal swab samples. Autofluorescence from the swab matrix significantly interfered with fluorescence polarization measurements. However; quantitative detection of H5-AIV was achieved by suppressing the matrix effect through 80-fold dilution. The limit of detection was 186.4 μg/mL. The assay can be completed within 20 minutes without complex sample preparation; making it suitable for rapid on-site applications; including field surveillance of AIV in wild birds and poultry. Furthermore; by designing appropriate tracers; the method can be extended to other AIV subtypes.
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3.3
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1.6W
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