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Determination of Shoot Regeneration Protocol in Aronia (Aronia melanocarpa (Michx.) Elliott) with New Generation Temporary Immersion Bioreactor System
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DOI：10.18016/ksutarimdoga.1644524.png)
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Abstract
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Aronia (Aronia melanocarpa) is an industrially important species belonging to the Rosaceae family. Among the berries, its importance has been increasing in recent years, and it is called a super fruit due to its effects on health. The purpose of this study was to compare the in vitro propagation of the 'Viking' aronia cultivar via the PlantForm bioreactor system, known as the new generation, with that via the approach. MS nutrient medium and cytokinin (BAP, KIN) sources (1.0, 2.0, and 4.0 mgL(-1)) were used in solid culture micropropagation and PlantForm bioreactor system trials. At the end of each subculture, the number of shoots (pcs/plantlet), plant height (cm), plant diameter (mm), number of leaves (pcs), plant fresh and dry weight (g) data, (cm), and root fresh and dry weight (g) data were examined in the micropropagation experiments in both systems. In terms of shoot vitality, the media containing KIN gave better results with 100% vitality rates in both systems. The PlantForm system yielded better plant height and fresh and dry weight results than the solid culture system. In fact, the highest value of 4.12 cm in plant height was obtained from MS+4.0 mgL(-1) KIN+0.01 mgL(-1) IAA medium. The best values in plant fresh and dry weight were determined in MS+2.0 mgL1 BAP+0.01 mgL(-1) IAA medium in the PlantForm system. In this study, the PlantForm bioreactor system was more effective for micropropagation than solid culture media.
Keywords:
Aronia
MS
PlantForm
Temporary Immersion Bioreactor
System
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