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Developing Blight-Tolerant American Chestnut Trees
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DOI：10.1101/cshperspect.a034587.png)
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Abstract
En 中文
An invasive fungal pathogen has reduced the American chestnut (Castanea dentata), once a keystone tree species within its natural range in the eastern United States and Canada, to functional extinction. To help restore this important canopy tree, blight-tolerant American chestnut trees have been developed using an oxalate oxidase-encoding gene from wheat. This enzyme breaks down oxalate, which is produced by the pathogen and forms killing cankers. Expressing oxalate oxidase results in blight tolerance, where the tree and the fungus can coexist, which is a more evolutionarily stable relationship than direct pathogen resistance. Genetic engineering (GE) typically makes a very small change in the tree's genome, potentially avoiding incompatible gene interactions that have been detected in some chestnut hybrids. The GE American chestnut also retains all the wild American chestnut's alleles for habitat adaptation, which are important for a forest ecosystem restoration program.
Keywords:
OXALATE OXIDASE GENE
AGROBACTERIUM-MEDIATED TRANSFORMATION
GERMIN-LIKE PROTEIN
ANTIMICROBIAL PEPTIDES
HYPOVIRULENT STRAINS
OXALIC-ACID
CRYPHONECTRIA-PARASITICA
CONSTITUTIVE EXPRESSION
ENDOTHIA-PARASITICA
ENHANCED RESISTANCE
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