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Development of a ratiometric N-CDs/RhB fluorescent probe for selective and sensitive detection of arginine and ascorbic acid
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J
DOI：10.1039/D6AY00293E.png)
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Abstract
En 中文
Arginine (Arg) and ascorbic acid (AA) are essential nutrients that significantly impact human health. In this research; we introduced a novel N-CDs/RhB ratiometric fluorescent probe for sensitive detection of Arg and AA. The probe was constructed through simple physical mixing of rhodamine B (RhB) and nitrogen-doped carbon dots (N-CDs); leveraging fluorescence resonance energy transfer (FRET) between N-CDs and RhB. The optimal excitation wavelength for N-CDs was determined to be 440 nm; with fluorescence emission at 510 nm; while RhB exhibited excitation and emission peaks at 557 nm and 572 nm; respectively. The N-CDs/RhB probe demonstrated excellent stability across various pH values and salt concentrations; and under UV light exposure; allowing reliable performance under diverse conditions. The probe exhibited high selectivity and anti-interference properties; with no significant response to other amino acids or food additives. Its response to Arg and AA was characterized by an “on–off–on” mechanism; with fluorescence quenching upon the addition of Arg due to electrostatic adsorption and static quenching; and fluorescence recovery upon the addition of AA through a redox reaction that weakened the guanidinium–carboxylate bridge interaction. The probe demonstrated linear detection ranges of 0.7–60 µM for Arg and 7.7–220 µM for AA; with detection limits of 0.21 µM and 2.32 µM; respectively. Real-world testing in functional beverages further confirmed the practical applicability of the probe; with recovery rates for Arg and AA ranging from 97.44% to 100.83%. These results highlight the potential of the N-CDs/RhB ratiometric fluorescent probe for real-time; precise sequential detection of Arg and AA in complex samples.
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