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Diffraction optical tomography using a quantitative phase imaging unit
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DOI：10.1364/OL.39.006935.png)
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Abstract
En 中文
A simple and practical method to measure three-dimensional (3-D) refractive index (RI) distributions of biological cells is presented. A common-path self-reference interferometry consisting of a compact set of polarizers is attached to a conventional inverted microscope equipped with a beam scanning unit, which can precisely measure multiple 2-D holograms of a sample with high phase stability for various illumination angles, from which accurate 3-D optical diffraction tomograms of the sample can be reconstructed. 3-D RI tomograms of nonbiological samples such as polystyrene microspheres, as well as biological samples including human red blood cells and breast cancer cells, are presented. (C) 2014 Optical Society of America
Keywords:
RED-BLOOD-CELLS
DIGITAL HOLOGRAPHIC MICROSCOPY
REFRACTIVE-INDEX
LIVING CELLS
LIVE CELLS
HEMOGLOBIN
MEMBRANE
INTERFEROMETRY
MORPHOMETRY
RESOLUTION
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