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Direct Identification of Polymer Chain Folding in Lamellar Crystals by Correlated Single-Molecule FRET and Atomic Force Microscopy
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DOI：10.1021/acs.macromol.6c01000.png)
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Abstract
En 中文
Polymer crystals often take the form of lamellae, which exhibit variations in thickness that are commonly attributed to heterogeneity in the state of chain folding. Here, single-molecule Förster resonance energy transfer (SM-FRET) microscopy and spatially correlated atomic force microscopy (AFM) height measurements were used to directly probe the correlation between chain conformation and local crystal thickness for end-labeled poly(ethylene oxide) chains. Correlated SM-FRET and AFM measurements on the same crystals revealed two dominant and well-defined populations: low FRET chains in thick lamellae (extended, n = 0) and high FRET chains in thin lamellae (singly folded, n = 1). Spatially resolved measurements showed that multiple folding states frequently coexist within individual crystals. Population-level analysis further revealed an intermediate FRET population, indicating additional conformational structure within crystalline regions. These results explicitly connect single-chain conformations with lamellar structure in polymer crystals and establish SM-FRET as a useful method for characterizing chain conformation.
Keywords:
Conformation
Crystals
Fluorescence resonance energy transfer
Polymers
Structural characteristics
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5.2
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3.6W
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9.4W
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