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Direct methylation sequencing
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DOI：10.1038/s41589-023-01356-9.png)
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Abstract
En 中文
Sodium bisulfite is widely used in methylation sequencing, yet it degrades DNA, and on its own, it does not discriminate methylated cytosine from its oxidized derivative, 5-hydroxymethylcytosine. A new bisulfite-free technique uses enzymatic modification of DNA for direct and accurate methylation mapping.
Keywords:
DNA
5-HYDROXYMETHYLCYTOSINE
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