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Directed evolution: the 'rational' basis for 'irrational' design

delete2000-08-01
delete126
PRE
AI
T
Tobin, MB *
C
Claes Gustafsson
G
Gjalt W. Huisman
DOI：10.1016/S0959-440X(00)00109-3delete
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Abstract

Abstract

En 中文
The development of powerful genetic manipulation formats has revolutionized the creation of functional biological molecules. Recent advances in directed evolution demonstrate that multiple properties of proteins can be optimized simultaneously and rapidly. Improved proteins often contain multiple and dispersed substitutions that act synergistically to improve enzyme properties and function. The benefits of such multiple changes are often not predictable from a priori structural knowledge. Furthermore, alternative solutions to gaining functional change can be obtained.
Keywords:
IN-VITRO EVOLUTION
MOLECULAR EVOLUTION
INCREMENTAL TRUNCATION
RANDOM MUTAGENESIS
PROTEIN EVOLUTION
ENZYMES
FAMILY
BIOCATALYSTS
ESTERASE
GENE

Journal

Current Opinion in Structural Biology cover
Current Opinion in Structural Biology
IF:
7
Papers:
3.8K
Citations:
1.3W

Organization

No organization information available