.png){kind=icon}
.png){kind=icon}
Return
Dynamics of Vibrio ostreicida in Mytilus galloprovincialis through in vivo infections
.png){kind=icon}
OA
AI
M
J
Y
M
K
M
L
D
L
DOI：10.3389/fimmu.2025.1727164.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
IntroductionVibrio species are widespread in coastal environments; where they are being increasingly associated with mortality episodes of farmed bivalves. This is the case for Vibrio ostreicida strain r172 isolated from a mortality event of adult mussels Mytilus galloprovincialis in Spain in 2022.Methods and ResultsIn this study; the infection dynamics and immune responses of adult M. galloprovincialis challenged with V. ostreicida r172 were investigated using different in vivo experimental approaches. First; a virulence assay by injection (1 x 108 CFU/100 µL) was performed at different temperatures (12; 18 and 24 °C). The results showed that mussel mortality (about 50 % within 8 days) was independent of increasing temperatures. Subsequently; an injection/cohabitation experiment was carried out placing together in the same tank V. ostreicida-injected (Donors; 108 CFU/mL) with un-injected mussels (Recipients). The time course of infection was then followed by evaluating positivity to V. ostreicida by PCR; responses of haemolymph components (haemocyte lysosomal membrane stability and serum lysozyme activity) and tissue histopathology (gills and digestive gland). The results showed a partial horizontal transfer of V. ostreicida from infected to uninfected mussels; with transient effects on haemolymph responses and histopathological lesions in both groups. Finally; in order to mimic more realistic environmental conditions; a bath infection experiment was carried out; exposing mussels to V. ostreicida in seawater (105 CFU/mL). This condition resulted in lower stress in haemocytes; moreover; no lysozyme release or histopathological alterations were observed.DiscussionOverall; the results show that M. galloprovincialis is able to cope with challenge with V. ostreicida; indicating that this Vibrio species is moderately pathogenic to adult mussels under the established experimental conditions.
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
5.9
Papers:
4.9W
Citations:
22.7W