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Eco-Friendly Fabrication of Gold Nanoparticles via Cabbage Extract for SPR-Based Colorimetric Detection of Biomolecules

delete2026-08-13
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S
Suhad. H. Mohsen *
S
Sohrab Ahmadi-Kandjani
DOI:10.1007/s11468-026-03407-ydelete
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Abstract

Abstract

En 中文
The present research proposes a novel, sustainable, and eco-friendly method for the synthesis of gold nanoparticles using *Brassica oleracea* (cabbage) extract, which were subsequently employed for biomolecule detection through a surface plasmon resonance (SPR)-based colorimetric approach. The formation of the nanoparticles was confirmed by UV–Vis spectroscopy, which exhibited an SPR peak at 544 nm for the uncoated AuNPs. Upon surface modification, the SPR peak shifted to 540 nm and 533 nm for the PVA- and PEG-coated AuNPs, respectively, indicating improved particle dispersion and reduced agglomeration. FTIR results confirmed the presence of characteristic bands at 3400–3600 cm⁻¹ (O–H) and 1630–1650 cm⁻¹ (C = O), demonstrating the role of biomolecules in both the reduction and stabilization of the nanoparticles. XRD analysis revealed a face-centered cubic (fcc) crystal structure with diffraction peaks indexed to the (111), (200), (220), and (311) planes, confirming the high crystallinity and phase purity of the synthesized AuNPs. TEM images showed particle sizes ranging from 11 to 59 nm depending on the stabilization method. In the bacterial detection experiment, the SPR peak of the uncoated AuNPs shifted from 520 to 529 nm after interaction with Pseudomonas aeruginosa, while the PVA- and PEG-coated AuNPs exhibited redshifts to 550 nm and 554 nm, respectively, accompanied by a distinct color change. Furthermore, fluorescence measurements revealed an increase in the emission intensity at 518 nm with increasing bacterial concentration. These findings demonstrate the efficiency of the developed system as a rapid, low-cost, and environmentally friendly biosensor with promising potential for diagnostic applications.
Keywords:
Eco-Friendly
Gold Nanoparticles
Cabbage Extract
Colorimetric Detection
Surface plasmon resonance

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