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Effects of sampling location and frequency on the detection of Mycoplasma gallisepticum populations in the respiratory tract of commercial layer pullets
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DOI：10.1016/j.psj.2026.107277.png)
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Abstract
En 中文
Mycoplasma gallisepticum (MG) is an economically important pathogen in layers, as it is responsible for chronic respiratory disease. Routine detection of MG is performed via swabbing of the trachea or choanal cleft. However, the possible impact of repeated sampling events on in vivo MG population dynamics within individual subjects has not been investigated. Therefore, eighty 11 wk-old Hy-Line W-36 layer pullets were infected with F-strain MG, and were individually caged in one of 4 identical rooms, with 20 birds per room. Two wk post-infection, 5 birds in each room were randomly assigned to either 2, 4, 8, or 16 d sampling interval schedules, and were maintained for an additional 32 d. At each sampling event, the birds were swabbed in the choanal cleft and trachea, and sample-associated MG populations were quantified using real-time PCR. A complete block experimental design was employed, with room as an experimental block containing multiple replicates of each sampling schedule. Data, within birds, was subjected to repeated measures analysis. Undetected bacterial populations were considered as a negative sample. There was no evidence of any difference in probability of detecting a positive sample or in trend over time of microbial abundance due to the different sampling schedules. However, there were ∼26 times as many genomic equivalents of MG in the choanal cleft than in the trachea across all the sampling intervals, and significantly greater probability of positivity in the choanal cleft at d 16 and d 32. It was concluded that the site of sampling influenced MG population with the sampling interval exerting no additional influence.
Keywords:
Choanal cleft
Layer, Mycoplasma gallisepticum, Sampling
Trachea
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