.png){kind=icon}
.png){kind=icon}
Return
Efficient expression of γ-DL-glutamyl hydrolase in Komagataella phaffii and its application in modification of poly-γ-glutamic acid from Bacillus velezensis
.png){kind=icon}
PRE
AI
H
Q
Y
X
江
DOI：10.1016/j.jbiotec.2026.04.023.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
• A γ-DL-glutamyl hydrolase (BvPgdS45) from B. velezensis was first expressed in K. phaffii. • The highest γ-DL-glutamyl hydrolase activity of 102.7 IU/mL was produced. • BvPgdS45 had great potential in regulation of γ-PGA molecular weight (MW) by in situ enzymatic hydrolysis. • A broad MW distribution (5.1×105 Da to 2.1×106 Da) and a high yield (62.6 g/L to 73.7 g/L) of γ-PGA were obtained in 5 L fermenter.
Keywords:
γ-DL-glutamyl hydrolase
Komagataella phaffii
poly-γ-glutamic acid
enzyme expression
molecular weight modification
Journal
IF:
3.9
Papers:
1.5W
Citations:
1.5W
Organization
Cited Papers