.png){kind=icon}
.png){kind=icon}
Return
Eggshell membranes as green carriers for Burkholderia cepacia lipase: A biocatalytic strategy for sustainable wastewater bioremediation
.png){kind=icon}
OA
AI
M
M
I
N
M
Z
N
N
V
S
DOI：10.1515/gps-2025-0099.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Early research on recycling agri-food industry waste focused on valorization to identify valuable components lost when waste is disposed of, reflecting a linear economy model. With the shift to a circular economy, current research aims for full recycling of waste containing high-value compounds. This study builds on previous work involving egg white layer proteins and high-purity calcium salts to explore the potential of eggshell membranes for lipase immobilization. Eggshell waste was treated with acids (5% hydrochloric, 10% acetic, 15% o-phosphoric), and then lipase from Burkholderia cepacia was immobilized using adsorption and covalent binding. The immobilized enzymes were tested for biochemical and operational properties. Results showed improved thermal stability, altered pH and temperature optima, and retention of up to 85% of initial activity after ten reuse cycles. Adsorption proved to be the most effective immobilization method, offering superior stability and reusability. Among the carriers tested, ESMC-HCl was identified as the most suitable, with the immobilized lipase successfully applied in the enzymatic pretreatment of wastewater from the oil-processing industry. This application achieved over 89% removal of chemical oxygen demand and reduced total oil content from 95% to 18% across four treatment cycles, demonstrating both effectiveness and reusability of the biocatalyst.
Keywords:
eggshell membrane
Burkholderia cepacia lipase
immobilization
wastewater bioremediation
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
G
IF:
3
Papers:
1.1K
Citations:
2.7K
Organization
Cited Papers