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Electrochemiluminescence Microscopy
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DOI：10.1002/anie.202407588.png)
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Abstract
En 中文
Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into an optical microscopy. The orthogonality of the electrochemical trigger and the optical readout distinguishes it from classic microscopy and electrochemical techniques, owing to its near-zero background, remarkable sensitivity, and absence of photobleaching and phototoxicity. In this minireview, we summarize the recent advances in ECL imaging technology, emphasizing original configurations which enable the imaging of biological entities and the improvement of the analytical properties by increasing the complexity and multiplexing of bioassays. Additionally, mapping the (electro)chemical reactivity in space provides valuable information on nanomaterials and facilitates deciphering ECL mechanisms for improving their performances in diagnostics and (electro)catalysis. Finally, we highlight the recent achievements in imaging at the ultimate limits of single molecules, single photons or single chemical reactions, and the current challenges to translate the ECL imaging advances to other fields such as material science, catalysis and biology. The orthogonality of the electrochemical trigger and the optical readout distinguishes electrochemiluminescence (ECL) from classic microscopy and electrochemical techniques. In this review, we summarize the recent advances in ECL microscopy, emphasizing original configurations which enable the mapping of the (electro)chemical reactivity and the imaging of biological entities. Finally, we highlight the recent achievements in imaging single ECL photons or molecules. image
Keywords:
electrochemistry
imaging
electrogenerated chemiluminescence
single molecule
microscopy
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