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Enabling methodology for high-throughput media screening in the context of multi-cell type co-cultures
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DOI：10.1016/j.jbiotec.2026.04.014.png)
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Abstract
En 中文
Recently, an increasing number of in vitro tissue models have been engineered, incorporating a growing variety of cell types more accurately reproduce native tissue architecture and function. However, optimisation of culture media to support those co-cultures remains challenging due to limited standardised protocols and scalable screening methods. Current co-culture approaches involve either direct cell-cell contact or indirect paracrine signalling through shared media, with each presenting distinct challenges regarding their characterisation. The corresponding commercial co-culture systems are often costly, lack modularity, and are unsuitable for high-throughput screening.
Keywords:
co-culture
high throughput screening
image analysis
media selection
tissue modelling
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