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Engineered CRISPR prime editors with compact, untethered reverse transcriptases
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DOI：10.1038/s41587-022-01473-1.png)
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Abstract
En 中文
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase (nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase (MMLV-RT). Here we show that separated nSpCas9 and MMLV-RT proteins function as efficiently as intact PE2 in human cells. We use this Split-PE system to rapidly identify and engineer more compact prime editor architectures that also broaden the types of RTs used for prime editing. A split prime editor architecture facilitates screening and engineering of improved variants.
Keywords:
TEMPLATE-PRIMER
IDENTIFICATION
GENERATION
NUCLEASES
DOMAIN