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Engineered crRNA for biosensing
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DOI：10.1016/j.tibtech.2024.06.006.png)
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Abstract
En 中文
CRISPR/Cas-based diagnostics (CRISPR-Dx) face challenges, including difficulty in detecting ultra short nucleotides, preamplification dependency, cross-contamination, insufficiency in on-pot detection paradigms, and inconvenience in detecting non-nucleic acid targets. This forum outlines the advances in engineered CRISPR RNA (crRNA) that address the aforementioned problems, highlighting challenges, opportunities, and future directions.
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14.9
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3.8K
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2.0W
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Cited Papers
CRISPR-Cas12a has both cis- and trans-cleavage activities on single-stranded DNA.png)
CELL RESEARCH
OAAI
CELL RESEARCHIF25.9
OAAIShareSaveFoldback-crRNA-Enhanced CRISPR/Cas13a System (FCECas13a) Enables Direct Detection of Ultrashort sncRNAANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSaveNew design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivityNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAIShareSaveRegulating the trans-Cleavage Activity of CRISPR/Cas12a by Using an Elongation-Caged Single-Stranded DNA Activator and the Biosensing ApplicationsANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSaveA PAM-free CRISPR/Cas12a ultra-specific activation mode based on toehold-mediated strand displacement and branch migrationNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAIShareSaveEnhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detectionNATURE COMMUNICATIONSOAAI
NATURE COMMUNICATIONSIF15.7
OAAIShareSaveEfficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNANUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAIShareSave
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