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Engineered Silver Nanoparticle Aggregate Hotspots on Glass Substrates for Ultrasensitive SERS Fingerprinting of Helicobacter pylori
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DOI：10.1007/s11468-026-03401-4.png)
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Abstract
En 中文
Surface-enhanced Raman spectroscopy (SERS) has emerged as a powerful plasmonic sensing strategy for rapid, label-free detection of biological targets via localized electromagnetic (EM) field enhancement at metallic nanostructures. In this work, plasmonically active silver nanoparticle (AgNP) aggregate films were fabricated on glass substrates via APTES-assisted surface functionalization followed by immersion coating. The AgNPs were synthesized using a modified Tollens reduction approach employing sucrose as a green reducing agent. Structural and optical characteristics of the resulting plasmonic substrates were investigated by UV–Vis spectroscopy, field emission scanning electron microscopy (FESEM), and transmission electron microscopy (TEM), confirming the formation of densely distributed nanoparticle aggregates capable of generating abundant SERS hotspots. The analytical performance of the substrates was evaluated for the detection of Helicobacter pylori, a clinically significant gastric pathogen. Distinct SERS fingerprints of H. pylori were successfully recorded over a concentration range of 101–1010 Colony-Forming Unit (CFU) mL− 1. The plasmonic substrate exhibited a limit of detection as low as 101 CFU mL− 1, an empirical enhancement factor (EF) of 4.49 × 106, and excellent signal reproducibility with a relative standard deviation of 6.31% obtained from five independent measurements. The observed sensitivity is attributed to strong localized surface plasmon resonance (LSPR) coupling within AgNP aggregates, resulting in efficient EM enhancement. These findings demonstrate that AgNP aggregate films deposited on glass provide a simple, low-cost, and highly reproducible plasmonic SERS platform for ultrasensitive bacterial detection and molecular fingerprinting, highlighting their potential for rapid biomedical sensing applications. Plasmonically active Ag nanoparticle aggregate films were fabricated on glass substrates. Dense nanoparticle aggregates generated abundant electromagnetic SERS hotspots. Distinct Raman fingerprints of Helicobacter pylori were detected without labeling. The plasmonic substrate achieved an ultrasensitive detection limit of 101 CFU mL-1. An enhancement factor of 4.49 × 106 and RSD of 6.31% demonstrated strong performance and reproducibility. The platform offers a simple and low-cost route toward plasmonic biosensing applications.
Keywords:
Surface-enhanced Raman spectroscopy (SERS)
Localized surface plasmon resonance (LSPR)
Silver nanoparticle aggregates
Plasmonic hotspots
Helicobacter pylori
Raman fingerprinting
Ultrasensitive detection
Glass-supported plasmonic substrate
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