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Engineering AraC L9K for Inducer-Independent pBAD Activation in Salmonella Gallinarum
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DOI：10.1016/j.jbiotec.2026.04.019.png)
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Abstract
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• AraC L9K mutation enables inducer-independent activation of the pBAD promoter • Single amino acid substitution stabilizes AraC in an active conformation • Constitutive GFP expression achieved in Salmonella Gallinarum without arabinose • Empty vector controls validate specificity of promoter activity • System provides a cost-effective tool for microbial biotechnology and therapeutic applications
Keywords:
AraC L9K
pBAD promoter
inducer-independent activation
Salmonella Gallinarum
GFP expression
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3.9
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1.5W
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1.5W
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