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Engineering multi-layered tissue constructs using acoustic levitation
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DOI：10.1038/s41598-019-46201-z.png)
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Abstract
En 中文
Engineering tissue structures that mimic those found in vivo remains a challenge for modern biology. We demonstrate a new technique for engineering composite structures of cells comprising layers of heterogeneous cell types. An acoustofluidic bioreactor is used to assemble epithelial cells into a sheet-like structure. On transferring these cell sheets to a confluent layer of fibroblasts, the epithelial cells cover the fibroblast surface by collective migration maintaining distinct epithelial and fibroblast cell layers. The collective behaviour of the epithelium is dependent on the formation of cell-cell junctions during levitation and contrasts with the behaviour of mono-dispersed epithelial cells where cell-matrix interactions dominate and hinder formation of discrete cell layers. The multilayered tissue model is shown to form a polarised epithelial barrier and respond to apical challenge. The method is useful for engineering a wide range of layered tissue types and mechanistic studies on collective cell migration.
Keywords:
RESPONSIVE CULTURE DISHES
AIRWAY EPITHELIAL-CELLS
ENDOTHELIAL-CELLS
IN-VITRO
EXPRESSION
MODEL
MANIPULATION
DYSFUNCTION
SPHEROIDS
HARVEST
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28.2W
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83.5W
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