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Engineering preparation and sustained delivery of bone functional exosomes-laden biodegradable hydrogel for in situ bone regeneration
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DOI：10.1016/j.compositesb.2023.110803.png)
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Abstract
En 中文
As an emerging cell-free therapy, exosomes derived from stem cells have been widely explored for tissue regeneration. However, it is still a great challenge to achieve the engineering extraction of bone-functionalized exosomes and their sustained delivery in tissue defects. Herein, a novel bone morphogenetic protein 2 (BMP2)-overexpressed engineered exosomes-loaded GelMA hydrogel was rationally constructed for in-situ bone regen-eration. BMP2-overexpressed exosomes were engineering prepared using mouse embryonic fibroblasts (NIH-3T3) as tool cells via gene manipulations and ultracentrifugation techniques. The sustained delivery of exosomes in cranial defects was achieved by loading GelMA biodegradable hydrogel. In addition, the pre-prepared BMP2-overexpressed exosome-loaded GelMA hydrogel showed excellent porous structure, rheological property and biocompatibility. In vitro biological results indicated that BMP2-overexpressed exosomes significantly promoted proliferation and osteogenic differentiation of bone mesenchymal stem cells (BMSCs). In vivo animal experiments analysis revealed BMP2-overexpressed exsomes-laden biodegradable hydrogel effectively improved in situ bone regeneration via mice cranial defect model. Overall, this study provided a promising cell-free therapy of engi-neered exosomes-loaded biodegradable hydrogel for treating bone defects.
Keywords:
Exosomes
Bone morphogenetic protein 2
Sustained delivery
Biodegradable hydrogel
In situ bone Regeneration
Journal
IF:
14.2
Papers:
1.2W
Citations:
8.9W