Engineering RNA-guided bridge recombinases for precise large-scale genome editing

doi:10.1016/j.tibtech.2026.07.025

Engineering RNA-guided bridge recombinases for precise large-scale genome editing

2026-08-20

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DOI：10.1016/j.tibtech.2026.07.025

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An RNA-guided bridge recombinase enables programmable, scarless chromosomal rearrangements in plant and mammalian genomes. Co-engineering the bridge RNA scaffold and a bacterial recombinase ISCro4 improves editing activity by up to 29.8-fold. The optimized system mediates rearrangements of up to 315 kb in rice and 31 kb in human cells without double-strand breaks. The compact ISCro4 system supports single-adeno-associated virus delivery for large-scale genome editing in mammalian cells. A 315-kb inversion rewires endogenous gene expression and generates herbicide-resistant rice.

Engineering RNA-guided bridge recombinases for precise large-scale genome editing

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