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Engineering structural variants to interrogate genome function
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DOI：10.1038/s41588-024-01981-7.png)
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Abstract
En 中文
Structural variation, such as deletions, duplications, inversions and complex rearrangements, can have profound effects on gene expression, genome stability, phenotypic diversity and disease susceptibility. Structural variants can encompass up to millions of bases and have the potential to rearrange substantial segments of the genome. They contribute considerably more to genetic diversity in human populations and have larger effects on phenotypic traits than point mutations. Until recently, our understanding of the effects of structural variants was driven mainly by studying naturally occurring variation. New genome-engineering tools capable of generating deletions, insertions, inversions and translocations, together with the discovery of new recombinases and advances in creating synthetic DNA constructs, now enable the design and generation of an extended range of structural variation. Here, we discuss these tools and examples of their application and highlight existing challenges that will need to be overcome to fully harness their potential. Structural variations (SVs) impact gene expression, genome stability and disease susceptibility. This Review discusses recent advances in genome-engineering tools that enable precise SV generation and highlights the challenges that remain.
Keywords:
SITE-SPECIFIC RECOMBINATION
REGULATORY ELEMENTS
CHROMOSOMAL DELETIONS
FLP-RECOMBINASE
CRE RECOMBINASE
MOUSE GENOME
CRISPR-CAS9
INTEGRATION
REVEALS
SYSTEM
Journal
IF:
40
Papers:
1.0W
Citations:
10.5W