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Enzymatic synthesis and characterization of chimeric isomaltomegalosaccharide via successive transglucosylation by C-terminally truncated dextran dextrinase
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DOI：10.1016/j.procbio.2026.06.008.png)
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Abstract
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• C-terminal truncation of dextran dextrinase alters product size distribution. • Isomaltomegalosaccharide yield increases by 39% as dextran formation decreases. • Truncation creates a kinetic bottleneck favoring intermediate chain elongation. • Mutant enzyme retains high α-(1→6) regiospecificity and catalytic efficiency.
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