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Establishment of A triplex TaqMan qPCR assay for EHP, VpTPD, VpAHPND with optimized efficacy using methyl cellulose

delete2026-04-24
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PRE
AI
Y
Yuzhi Wang
C
Chuang Cui
X
Xiaoqian Tang *
J
Jing Xing
X
Xiuzhen Sheng
H
Heng Chi
W
Wenbin Zhan
DOI:10.1016/j.jip.2026.108639delete
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Abstract

Abstract

En 中文
• Developed a triple TaqMan qPCR method for the detection of EHP, VpTPD, VpAHPND. • Confirmed presence of multi-target interaction effects in multiplex TaqMan qPCR. • Methyl cellulose could significantly enhance amplification efficiency.
Keywords:
EHP
VpTPD
VpAHPND
triplex TaqMan qPCR
methyl cellulose

Journal

Journal of Invertebrate Pathology cover
Journal of Invertebrate Pathology
IF:
2.4
Papers:
3.7K
Citations:
7.3K

Organization

O
Ocean University of China
Scholars:
3.3K
Papers: 1.1K
Citations: 2.6W
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