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Exploiting nanoprobe X-ray techniques for imaging of biomineralisation; chemical; structural and in situ opportunities

delete2025-09-30
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J
Jessica M. Walker
M
Miguel A. Gomez‐Gonzalez
J
Johannes Ihli
J
Julia E. Parker *
DOI:10.1039/D5FD00037Hdelete
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Abstract

Abstract

En 中文
Advances in X-ray nanoprobe beamlines at synchrotrons across the world present exciting opportunities for rich multimodal imaging of biomineral structures and their formation processes. The combination of techniques provides a sensitive probe of both chemistry and structure; making X-ray nanoprobes an important tool for investigating crystallite growth and orientations; interfaces; and assembly of building blocks into hierarchical structures. A discussion of these capabilities is presented with reference to recent examples using a range of nanoprobe imaging techniques for investigating enamel structure; as well as coccolith properties. Key opportunities for the use of X-ray nanoprobes lie in exploiting the penetrating power and coherence properties of synchrotron X-rays in order to image in situ processes or apply coherent diffractive imaging techniques to obtain higher resolutions. To this end initial results demonstrating the observation of calcium phosphate mineralisation; in a liquid environment; using nano-X-ray fluorescence mapping are presented; and the role of X-ray dose and beam induced effects is considered. Finally novel results from tomographic ptychography imaging of Mytilus edulis mussel shell calcite prisms are discussed; where the segmentation of the phase density into organic and mineral content gives insights into the mechanisms underlying mineral prism formation and the role of the organic matrix in biomineralisation.
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Faraday Discussions cover
Faraday Discussions
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