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Exploration of bacteria isolated from black soldier fly larvae for plant growth-promotion and organic acid production
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DOI：10.2306/scienceasia1513-1874.2026.040.png)
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Abstract
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Bacteria isolated from Black Soldier Fly (BSF) larvae have significant potential as effective sources of beneficial microorganisms. The objective of this research was to investigate and classify the utility of bacteria isolated from the BSF larvae. The isolation process involved examining the microorganisms' morphology and performing Gram staining, followed by classification using the 16S rRNA gene. The activities of the isolates-including ammonium release, phosphate solubilization, plant hormone production, and organic acid generation-were thoroughly analyzed. A total of twelve bacterial isolates were obtained from the BSF larvae. Selected isolates with notable activity levels and non-pathogenic characteristics were subjected to further investigation. Among these, isolate BSFL07 was identified as Bacillus siamensis, which produced 25.12 Iig/ml of indole-3-acetic acid (IAA) and exhibited the highest production of isovaleric acid (22.73 Iig/ml). Isolate BSFL08 was identified as Bacillus stercoris, producing 14.38 Iig/ml of IAA. Isolate BSFL11 was classified as Bacillus wiedmannii, producing 18.96 Iig/ml of IAA, and the highest levels of acetic acid (137.1 Iig/ml) and isobutyric acid (21.08 Iig/ml). All three isolates exhibited phosphate-solubilizing activity and limited nitrogen-fixation ability, which are key traits associated with plant growth promotion. Future studies will evaluate the effects of growth stage, cultivation time, and medium C/N ratio on metabolite production to optimize their application as biofertilizers.
Keywords:
organic acid
black soldier fly
nitrogen fixation
plant growth-promoting bacteria
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