.png){kind=icon}
.png){kind=icon}
Return
Exploring salinity induced adaptations in marine diatoms using advanced photonic techniques
.png){kind=icon}
OA
AI
DOI：10.1038/s41598-024-83640-9.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Photonic-based methods are crucial in biology and medicine due to their non-invasive nature, allowing remote measurements without affecting biological specimens. The study of diatoms using advanced photonic methods remains a relatively underexplored area, presenting significant opportunities for pioneering discoveries. This research provides a comprehensive analysis of marine diatoms, specifically Nitzschia sp., across varying salinity levels, integrating fluorescence lifetime imaging microscopy (FLIM), combined photoacoustic and fluorescence tomographies (PAFT), and ultrastructural examinations using transmission electron microscopy. Key findings include a systematic shift in the mean fluorescence lifetime from 570 ps at 20 parts per thousand to 940 ps at 80 parts per thousand, indicating functional adaptations in chlorophyll molecules within light-harvesting complexes. At 60 parts per thousand salinity, anomalies are observed in the development of silica valves and polysaccharide layers, suggesting abnormalities in valve morphogenesis. Lipid droplets within the cells display a minimum diameter at 40 parts per thousand, indicating metabolic adjustments to osmotic stress. The intensity of both fluorescence and photoacoustic signals increases with increasing salinity levels. These insights enhance understanding of the ecological implications of salinity stress on diatom communities and pave the way for future research on leveraging the unique adaptive mechanisms of microalgae for environmental monitoring and sustainable biotechnological applications.
Keywords:
Fluorescence lifetime imaging
Photoacoustic imaging
Ultrastructure
Salinity
Diatoms
Journal
IF:
3.9
Papers:
27.1W
Citations:
83.5W
View more organizations