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Exploring the Latent Mechanism of Gualou Xiebai Banxia Decoction for Anti-Myocardial Ischemia by Systems Pharmacology and Animal Experiment
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DOI：10.1177/1934578X261446661.png)
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Abstract
En 中文
BackgroundMyocardial ischemia, characterized by insufficient blood flow to the heart muscle, causes significant heart-related complications and mortality. Current treatments have side effects, necessitating novel therapeutic strategies. Gualou Xiebai Banxia Decoction (GXBD), a widely used Traditional Chinese Medicine decoction, shows promise but its active constituents and molecular mechanisms remain unclear. To explore GXBD's mechanism in treating myocardial ischemia using systems pharmacology.MethodsActive compounds were selected from TCMSP database based on pharmacokinetic parameters. Compound-targets were predicted and hub targets identified through degree centrality analysis. Molecular docking validated interactions. Networks were constructed to explore anti-myocardial ischemia mechanisms. A myocardial ischemia-like animal model was established with heart tissue pathology observation and ELISA for key targets confirmation.ResultsWe identified 34 active compounds interacting with 169 related hub targets. GXBD modulates response to stimulus and biological regulation through signaling pathways. Network analysis revealed GXBD's multi-targeting nature. Top 10 targets identified through PPI network: AKT1, TNF, INS, EGFR, IL1B, MAPK3, PTGS2, MMP9, CCL2 and PPARG. Myocardial ischemia-like rats showed cardiac muscle cells disordered, inflammatory cell infiltration, and significantly increased MMP9, IL-1 beta, TNF-alpha, EGFR-all reversed by 5.6g/kg GXBD.ConclusionsGXBD demonstrates multi-target therapeutic potential with holistic patient care approach. Systems pharmacology revealed GXBD's pharmacological profile, suggesting anti-myocardial ischemia effects via regulating AKT1, TNF, INS, EGFR, IL1B, MAPK3, PTGS2, MMP9, CCL2 and PPARG. This investigation offer essential insights to clarify the bioactive constituents and cardioprotective mechanism of GXBD in myocardial ischemia.
Keywords:
myocardial ischemia
Gualou Xiebai Banxia decoction
systems pharmacology
animal experiments
molecular mechanisms
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