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Extracellular matrix regulates force transduction at VE-cadherin junctions
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DOI：10.1091/mbc.E22-03-0075.png)
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Abstract
En 中文
Increased tension on VE-cadherin (VE-cad) complexes activates adaptive cell stiffening and local cytoskeletal reinforcement-two key signatures of intercellular mechano-transduction. Here we demonstrate that tugging on VE-cad receptors initiates a cascade that results in downstream integrin activation. The formation of new integrin adhesions potentiates vinculin and actin recruitment to mechanically reinforce stressed cadherin adhesions. This cascade differs from documented antagonistic effects of integrins on intercellular junctions. We identify focal adhesion kinase, Abl kinase, and RhoA GTPase as key components of the positive feedback loop. Results further show that a consequence of integrin involvement is the sensitization of intercellular force transduction to the extracellular matrix (ECM) not by regulating junctional tension but by altering signal cascades that reinforce cell-cell adhesions. On type 1 collagen or fibronectin substrates, integrin subtypes alpha 2 beta 1 and alpha 5 beta 1, respectively, differentially control actin remodeling at VE-cad adhesions. Specifically, ECM-dependent differences in VE-cad force transduction mirror differences in the rigidity sensing mechanisms of alpha 2 beta 1 and alpha 5 beta 1 integrins. The findings verify the role of integrins in VE-cad force transduction and uncover a previously unappreciated mechanism by which the ECM impacts the mechanical reinforcement of interendothelial junctions.
Keywords:
SHEAR-STRESS
CELL-CELL
INTEGRIN ACTIVATION
ALPHA-CATENIN
MECHANOTRANSDUCTION
ADHESION
STIFFNESS
FIBRONECTIN
TENSION
PHOSPHORYLATION
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