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Fabrication of Buoyancy-Assisted Separable HGM@SiO2-NH2 Composite and its Application in Protein Adsorption-Desorption
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DOI：10.1142/S1793292026501079.png)
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Abstract
En 中文
To address the need for efficient and readily separable adsorbents in protein purification, this study developed a novel amino-functionalized silica-coated hollow glass microsphere composite (HGM@SiO2-NH2) using commercially available hollow glass microspheres (HGM) as the substrate. This material integrates the buoyancy of HGM with a functional silica-amine shell designed for electrostatic protein capture. Characterization via scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta potential measurement, Fourier transform infrared spectroscopy (FTIR) and acid-base titration confirmed the successful layered construction of the material, revealing a surface amino group density of 15.99mmol/g along with excellent dispersion and buoyancy-driven separation characteristics in aqueous media. Adsorption experiments using bovine serum albumin (BSA) as a model protein demonstrated a high adsorption capacity of 43.7mg/g. The adsorption process was rapid, reaching approximately 38mg/g (about 86% of the equilibrium capacity) within 30min, and was primarily governed by electrostatic interactions, as evidenced by optimal performance at pH 6.5. Kinetic studies showed strong adherence to the pseudo-second-order kinetic model, while equilibrium data fitted well with the Langmuir isotherm model, indicating monolayer adsorption onto homogeneous sites. Furthermore, efficient desorption (84.5%) was achieved within 30min using a high-concentration salt solution. The material also exhibited good stability over multiple cycles, retaining approximately 70% of its initial adsorption capacity after six consecutive adsorption-desorption cycles. This work demonstrates that HGM@SiO2-NH2 is a promising and reusable adsorbent platform that combines efficient protein binding via electrostatic interactions with convenient buoyancy-assisted separation. These findings lay a solid foundation for its potential application in bioseparation processes.
Keywords:
Hollow glass microspheres
amino-functionalized
protein adsorption
electrostatic interaction
buoyancy-assisted separation
reusable adsorbent
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