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Flanking-Mismatch Engineered CRISPR-Cas12a Integrated with LAMP for Portable SNP Discrimination
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DOI：10.1016/j.snb.2026.140663.png)
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Abstract
En 中文
• Developed a paired flanking-mismatch CRISPR-Cas12a/LAMP workflow for programmable SNP discrimination • Demonstrated position-dependent amplification of apparent Cas12a activity differences between WT and MT targets • Achieved a discrimination factor of 13.36 with reliable TP53 variant-allele discrimination down to 5% VAF • Demonstrated preliminary feasibility using sequencing-confirmed Rett syndrome serum specimens carrying MECP2 c.502 C>T mutations • Confirmed cross-platform kinetic consistency on a portable fluorescence analyzer for near-patient SNP testing
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