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Fluorescent coherent diffractive imaging with accelerating light sheets
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DOI：10.1364/OE.27.013015.png)
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Abstract
En 中文
Fluorescence microscopy is a powerful method for producing high fidelity images with high spatial resolution, particularly in the biological sciences. We recently introduced coherent holographic image reconstruction by phase transfer (CHIRPT), a single-pixel imaging method that significantly improves the depth of field in fluorescence microscopy and enables holographic refocusing of fluorescent light. Here we demonstrate that by installing a confocal slit conjugate to the illuminating light sheets used in CHIRPT, out-of-focus light is rejected, thus improving lateral spatial resolution and rejecting noise from out-of-focus fluorescent light. Confocal CHIRPT is demonstrated and fully modeled. Finally, we explore the use of beam shaping and point-spread-function engineering to enable holographic single-lens light-sheet microscopy with single-pixel detection. (C) 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
Keywords:
MICROSCOPY
RECONSTRUCTION
Journal
IF:
3.3
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6.1W
Citations:
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