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Fluorescent sensor for bacterial recognition
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DOI：10.1016/j.saa.2013.02.041.png)
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Abstract
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Boronic acid-based fluorescent sensor is one of the non-enzymatic methods used for the recognition of saccharides. Since bacterial membrane has polysaccharides with dial groups, boronic acids probe could be applied for rapid bacterial recognition. Escherichia coli (XL-1 blue) were recognized by applying (3-(5-(dimethylamino) naphthalene-1-sulfonamide) phenyl) boronic acid (DNSBA) as a sensor and the fluorescence recorded by fluorometer micro-plate reader. Results showed that, fluorescence records of DNSBA increase in a dose dependent manner upon increasing the bacterial cell numbers. Moreover, the increase in the number of bacterial cells induces a shift in the spectra due to the formation of the anionic form of boronic acid complex. Therefore, DNSBA is an efficient sensor for monitoring bacterial cells. (c) 2013 Elsevier B.V. All rights reserved.
Keywords:
Boronic acid
Sensor
Bacterial detection
Fluorescence
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