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Force versus Response: Methods for Activating and Characterizing Mechanosensitive Ion Channels and GPCRs
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DOI：10.1002/adhm.202402167.png)
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Abstract
En 中文
Mechanotransduction is the process whereby cells convert mechanical signals into electrochemical responses, where mechanosensitive proteins mediate this interaction. To characterize these critical proteins, numerous techniques have been developed that apply forces and measure the subsequent cellular responses. While these approaches have given insight into specific aspects of many such proteins, subsequent validation and cross-comparison between techniques remain difficult given significant variations in reported activation thresholds and responses for the same protein across different studies. Accurately determining mechanosensitivity responses for various proteins, however, is essential for understanding mechanotransduction and potential physiological implications, including therapeutics. This critical review provides an assessment of current and emerging approaches used for mechanosensitive ion channel and G-Coupled Receptors (GPCRs) stimulation and measurement, with a specific focus on the ability to quantitatively measure mechanosensitive responses. This review explores techniques used to examine mechanosensitive ion channels and GPCRs, which convert mechanical signals into cellular responses. While these methods have provided valuable insights, discrepancies in reported activation thresholds and responses complicate comparison. The article critically assesses established and emerging approaches for measuring mechanosensitivity, highlighting challenges and opportunities. image
Keywords:
G-Coupled Receptors
ion channels
mechanotransduction
mechanosensitive
patch-clamp
stimulation
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