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Genomes by design
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DOI：10.1038/nrg3956.png)
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Abstract
En 中文
Next-generation DNA sequencing has revealed the complete genome sequences of numerous organisms, establishing a fundamental and growing understanding of genetic variation and phenotypic diversity. Engineering at the gene, network and whole-genome scale aims to introduce targeted genetic changes both to explore emergent phenotypes and to introduce new functionalities. Expansion of these approaches into massively parallel platforms establishes the ability to generate targeted genome modifications, elucidating causal links between genotype and phenotype, as well as the ability to design and reprogramme organisms. In this Review, we explore techniques and applications in genome engineering, outlining key advances and defining challenges.
Keywords:
UNNATURAL AMINO-ACIDS
SEQUENCE-SPECIFIC ANTIMICROBIALS
EXPANDED GENETIC-CODE
CRISPR-CAS NUCLEASES
ESCHERICHIA-COLI
DIRECTED EVOLUTION
IN-VIVO
SYNTHETIC BIOLOGY
TRANSFER-RNA
PROTEIN DESIGN
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