.png){kind=icon}
Return
Gigapixel imaging with a novel multi-camera array microscope
.png){kind=icon}
OA
AI
DOI：10.7554/eLife.74988.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
The dynamics of living organisms are organized across many spatial scales. However, current cost-effective imaging systems can measure only a subset of these scales at once. We have created a scalable multi-camera array microscope (MCAM) that enables comprehensive high-resolution recording from multiple spatial scales simultaneously, ranging from structures that approach the cellular scale to large-group behavioral dynamics. By collecting data from up to 96 cameras, we computationally generate gigapixel-scale images and movies with a field of view over hundreds of square centimeters at an optical resolution of 18 mu m. This allows us to observe the behavior and fine anatomical features of numerous freely moving model organisms on multiple spatial scales, including larval zebrafish, fruit flies, nematodes, carpenter ants, and slime mold. Further, the MCAM architecture allows stereoscopic tracking of the z-position of organisms using the overlapping field of view from adjacent cameras. Overall, by removing the bottlenecks imposed by single-camera image acquisition systems, the MCAM provides a powerful platform for investigating detailed biological features and behavioral processes of small model organisms across a wide range of spatial scales.
Keywords:
microscopy
computational optics
computational imaging
behavioral analysis
Physarum polycephalum
Camponotus pennsylvanicus
calcium imaging
C
elegans
D
melanogaster
Zebrafish
Journal
IF:
0
Papers:
1.8W
Citations:
16
Organization
Cited Papers
ShareSaveShareSaveRetinomorphic Event-Based Vision Sensors: Bioinspired Cameras With Spiking Output.png)
PROCEEDINGS OF THE IEEE
OAAI
PROCEEDINGS OF THE IEEEIF25.9
OAAIShareSaveProbabilistic Models of Larval Zebrafish Behavior Reveal Structure on Many ScalesCURRENT BIOLOGYOAAI
CURRENT BIOLOGYIF7.5
OAAIShareSaveShareSaveHigh numerical aperture Fourier ptychography: principle, implementation and characterizationOPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAIShareSaveAdaptive Scanning Optical Microscope (ASOM): A multidisciplinary optical microscope design for large field of view and high resolution imagingOPTICS EXPRESSOAAI
OPTICS EXPRESSIF3.3
OAAIShareSaveCharacterization of Proprioceptive System Dynamics in Behaving Drosophila Larvae Using High-Speed Volumetric MicroscopyCURRENT BIOLOGYOAAI
CURRENT BIOLOGYIF7.5
OAAIShareSave
View more