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Growing bacterial cellulose under AC electric fields
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DOI：10.1093/oxfmat/itaf007.png)
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Abstract
En 中文
Dielectrophoresis, or DEP, is an established technique used to physically manipulate particles by exploiting their inherent interaction with a non-uniform electric field. The strength and direction of the DEP force on a particle is dependent on the frequency of the applied field and the dielectric properties of the bacterial cell and suspending media. Komagataeibacter xylinus are bacteria that can synthesize a fibrous, web-shaped organic material called bacterial cellulose (BC) with desirable properties such as increased crystallinity and high mechanical strength. There is also potential for the controlled and enhanced growth of BC by combining DEP techniques with K. xylinus towards structuring BC as it is grown. To this end, two fundamental questions are tackled in this study: what is the response of K. xylinus to a field of varying frequencies, i.e. alternating current or AC? And will K. xylinus produce cellulose under the conditions envisioned for DEP manipulation in a microreactor? A titanium-based semicircular microelectrode chip was used to study the impact of AC electrostimulation on BC synthesis. Results show that K. xylinus can be trapped using DEP at frequencies from 250 kHz to 10 MHz at a polarization voltage of 5 Vpp, with the greatest cell capture and growth over 96 h occurring at 500 kHz; and that synthesis of BC is increased at high field strengths, up to 54 kV/m, over an incubating period up to 14 days.
Keywords:
microbial printer
dielectrophoresis
K. xylinus
electro synthesis
electro fermentation
Journal
O
IF:
4.5
Papers:
45
Citations:
267
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